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Rapid Gram Stain

Release time:2016-8-12      Reads:9800

Rapid Gram Stain

Intended use:

A rapid staining procedure to classify bacteria based on their cell wall characteristic.  According to the stain results, bacteria can be divided into two different groups - Gram positive (purple) and Gram negative (red).

 

Principle:

Gram stain was devised empirically by Christian Gram, a Danish physician, in 1884. When bacteria are stained with certain basic dyes (such as the crystal violet in Rapid Gram Stain), those cells with high peptidoglycan contents (Gram-positive) in their cell wall will resist decolorization with organic solvents such as ethanol or acetone, while the Gram-negative species (with a thin layer of peptidoglycan in the cell wall) can be easily decolorized.

 

Methods:

1. Stain the sample slide with Crystal Violet for 10 seconds. Rinse with water gently, and then remove the

    excess water.

2. Apply Iodine Solution for 10 seconds. Rinse with water gently, and then remove the excess water.

3. Add Decolorizer for 10~20 seconds. Rinse with water gently, and then remove the excess water.

4. Counterstain with Safranin Solution for 10 seconds. Rinse with water gently.

5. Blot up sample slide with bibulous paper (or allow to air dry), and then examine the slide under a microscope.

 

Specifications:

Contents

4vialsx20ml/kit

4Btlsx100ml/kit

4Btlsx250ml/kit

4x(6Btlsx500ml)/kit

Components

Crystal Violet

1x20ml

1x100ml

1x250ml

6x500ml

Crystal Violet, Ethanol

Iodine Solution

1x20ml

1x100ml

1x250ml

6x500ml

Iodine, Potassium iodide

Decolorizer

1x20ml

1x100ml

1x250ml

6x500ml

Acetone, Ethanol

Safranin Solution

1x20ml

1x100ml

1x250ml

6x500ml

Safranin, Ethanol

 

Precaution:

1. The length of stain will depend on the specimen type and smear thickness, etc. For female leucorrhea

    smear, staining time should be slightly longer (more than 10 seconds) for better results.

2. Cap reagent bottle immediately after each use to prevent reagent evaporation.

3. Do not use any reagent beyond the stated expiration date. For kit storage, avoid exposure to extreme high

    or low temperature and sunlight.

 

Expected Results:

Gram-positive organisms appear purple.

Gram-negative organisms appear red.

 

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