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Periodic Acid-Schiff (PAS) Stain

Release time:2016-8-5      Reads:4085

Periodic Acid-Schiff (PAS) Stain

Intended Use:
This kit is for staining bone marrow and blood cellsmear.

Principle:
PAS Stain is based on the principle of periodic acid-Schiff reaction. Periodic acid can oxidize Ethylene Glycol

(-CHOH-CHOH) contained in glycogen polysaccharides of cells to turn into dialdehyde (-CHO-CHO).

Subsequently, aldehyde will react with colorless Fucshin to form a red-fuchsin compound incytoplasm.


Specifications:

Contents

5Tests/Kit

20Tests/Kit

100Tests/Kit

Components

Fixative

(solution A)

1vial×5ml

1vial×20ml 

1vial×100ml

Ethanol

Periodic Acid

(solution B)

1vial×5ml 

1vial×20ml 

1vial×100ml 

Periodic Acid

Schiff's Reagent

(solution C)

1vial×5ml

1vial×20ml

1vial×100ml

Fuchsin Basic

Hematoxylin

(solution D)

1vial×5ml 

1vial×20ml 

1vial×100ml 

Hematoxylin



Methods:
1. Dry the smear. Fix smear in solution A for 30 seconds and rinse with distilled water. Air dry or dry with

    absorbent paper.


2. Drop solution B on slide to fully cover the smear for 5minutes. Rinse with distilled water and dry with filter

    paper or dry in the air.


3. Drop solution C on slide to fully cover the smear for 10~15 minutes. Rinse with distilled water for 5 minutes.


4. Counterstain with solution D for 1~2 minutes. Rinsewith distilled water. Dry the smear for microscopic

    examination.

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